SHORT ALCOHOL CONSUMPTION IMPAIRS THE OFFSPRING HEALTH IN MICE

GOTFRYD L.; STINSON M. G. ; ALCAIN J.; VERMEULEN M; VANZULLI S; CAMBIASSO M ; NAGUILA Z ; ERLEJMAN A.G.; CALVO J.C.; SALOMONE G.; VANINA F.

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Congreso; International Federation of Placenta Associations (IFPA 2019).Placenta: the origin of pregnancy health and disease; 2019

International Federation of Placenta Associations

Previously, we observed that male alcohol consumption delayed embryo differentiation by deregulating peri-implantation events and altering embryo trophoblast and inner cell mass morphology in vitro. Objective: To evaluate the effects of paternal alcohol consumption on the offspring in mice. Methods: CF-1 male mice were exposed (treated group) or not (control group) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. Control and treated males were mated with non-treated CF-1 females (1:1), and pregnant females (vaginal plug: day1) were determined. Pregnancy outcome was evaluated and litter mortality, weight and size registered. Blood from adult offspring was analyzed in a Coulter counter. Then, they were sacrificed and dissected. Offspring´s spleen was weighed and analyzed histologically. Spleen cell populations were determined by flow cytometry. Results: After 21 days of pregnancy, born mice from both groups were registered and, during the first week after birth, an increased number of deaths from treated group (p