INVESTIGADORES
ERLEJMAN Alejandra Giselle
artículos
Título:
The Interaction of Flavonoids with Membranes: Potential Determinant of Flavonoid Antioxidant Effect
Autor/es:
ERLEJMAN A.G.; FRAGA CG.; OTEIZA P.I.
Revista:
FREE RADICAL RESEARCH
Editorial:
Taylor & Francis Group Ltd.
Referencias:
Lugar: Inglaterra; Año: 2004 vol. 38 p. 1311 - 1320
ISSN:
1071-5762
Resumen:
Twenty six phenolic substances including representatives
of the families, flavanones, flavanols and procyanidins,
flavonols, isoflavones, phenolic acids and phenylpropanones
were investigated for their effects on lipid
oxidation, membrane fluidity and membrane integrity. The
incubation of synthetic phosphatidylcholine (PC) liposomes
in the presence of these phenolics caused the
following effects: (a) flavanols, their related procyanidins
and flavonols were the most active preventing 2,20-azo-bis
(2,4-dimethylvaleronitrile) (AMVN)-induced 2-thiobarituric
acid-reactive substances (TBARS) formation,
inducing lipid ordering at the water-lipid interface, and
preventing Triton X-100-induced membrane disruption;
(b) all the studied compounds inhibited lipid oxidation
induced by the water-soluble oxidant 2,20-azo-bis
(2-amidinopropane) (AAPH), and no family-related effects
were observed. The protective effects of the studied
phenolics on membranes were mainly associated to
the hydrophilicity of the compounds, the degree of
flavanol oligomerization, and the number of hydroxyl
groups in the molecule. The present results support
the hypothesis that the chemical structure of phenolics
conditions their interactions with membranes. The interactions
of flavonoids with the polar head groups of
phospholipids, at the lipidwater interface of membranes,
should be considered among the factors that contribute to
their antioxidant effects.