Characterizaton of a new Xanthomonas citri subsp. citri isolate which triggers a host-specifc response

ROESCHLIN R.A.; CHIESA M.A.; ENRIQUE R.; FAVARO M. A.; TORRES P.S.; FILIPPONE M.P.; GMITTER JR, F.G.; VOJNOV, A.A.; CASTAGNARO A. P.; MARANO M.R.; GADEA VACAS J.

Valencia

Congreso; XII International Citrus Congress; 2012

Citrus is an economically important fruit crop that is severely aficted by Asiatc Citrus Bacterial Canker (CBC), a disease caused by Xanthomonas citri subsp. citri (XC). Recently, we have identfed a XC strain, named XCT44, which induces a hypersensitve-like response in Citrus limon leaves. To gain insight into the dynamics of these host-specifc responses, we have investgated bioflm formaton and accumulaton of defense markers by XCT44 on diferent citrus species. Bioflm formaton was monitored by confocal laser scanning microscopy (CLSM) over a 10-day period. XCT44 strain was unable to form structured bioflm afer 6 days post-inoculaton. This observaton is associated with early producton of reactve oxygen species by the plant. Transcriptomic analysis of C. limon leaves in response to the non pathogenic (XCT44) and pathogenic (XC) strains showed that XCT44 induces specifc genes that points towards the involvement of salicylic acid and glucosinolates metabolism in the onset of the defense responses. Further characterizaton of these genes with a role in this HR-like response will be important for the development of new strategies to manage the disease.