INVESTIGADORES
ALLEMANDI Daniel Alberto
congresos y reuniones científicas
Título:
Neuroprotective effect of melatonin loaded in ethylcellulose nanoparticles applied topically in a retinal degeneration model in rabbits
Autor/es:
CAROLINA DEL V. BESSONE, ; SOFÍA M. MARTINEZ, ; JOSÉ D. LUNA, ; MARILYN A. MÁRQUEZ, ; DANIEL A. ALLEMANDI, ; ÁGATA R. CARPENTIERI, ; DANIELA A. QUINTEROS
Lugar:
Villa Carlos Paz
Reunión:
Congreso; XXXIV Reunión Anual SAN 2019. Sociedad Argentina de investigación en Neurociencias; 2019
Institución organizadora:
Sociedad Argentina de investigación en Neurociencias
Resumen:
During the development of different ocular pathologies, such as glaucoma, oxidative stress becomes the main cause of cellular damage. The antioxidant capacity of the cell is insufficient to protect the retinal ganglion cells (RGCs). Therefore, the exogenous administration of antioxidant agents is a promising strategy to inhibit some of the steps involved in the death ofretinal cells. Melatonin (ME) has been described as being an effective antioxidant in the retina with direct and indirect free radical scavenging properties, and being able to protect the photoreceptor outer segment membranes from a free radical attack induced by light. This project describes the elaboration of nanocapsules (NCECMEs) containing ME, and includes theformulation, physicochemical characterization, in vitro drug release, transcorneal permeation studies and an in vivo study of irritation. The neuroprotective effect of ME was also evaluated using the induced retinal degeneration (RD) model. In vitro ME release (1 and 2 mg/mL) fromNCECMEs was found to be slower than ME solution. However, ex vivo assays demonstrated a higher permeation, attributable to the extended residence time of NCECMEs and also an ME effect on the membrane at low concentrations, leading to an increase in drug absorption.NCECMEs appeared to be more efficient for RGC protection, compared to ME solutions, against induced RD model, as indicated by damage indicators such as the apoptotic index (AI) and retina integrity.