Preliminary assessment of three plant extracts to obtain silver nanoparticles for their application as biocides to coatings

L. BERBERIA ROQUE; E. GÁMEZ ESPINOSA; M. VIERA; N. BELLOTTI

Manchester

Simposio; 17th International Biodeterioration & Biodegradation Symposium; 2017

International Biodeterioration Biodegradation Society

In the last decades the interest in the study of the biodeterioration in buildings has increased due to the consequences that it generates in indoor environment and Human health. The control of biodeterioration has led to the development of new biocides for the application in coatings formulations among which are the nanoparticles. Green synthesis of nanoparticles based on the use of aqueous plant extracts is promising, it is accessible, effective, low cost and eco-friendly. The aim of this research was to carry out a preliminary assessment of the use of aqueous extracts from three native argentine plants: Ilex paraguariensis Saint Hilaire, Schinus molle and Equisetum giganteum for the synthesis of silver nanoparticles (NpAg).The plant extracts obtained with Mili-Q water at 80°C were using to synthesized NpAg with AgNO3 solutions, 10-1M and 10-2M at pH = 7 and 60°C. UV-vis spectroscopy was carry on to confirm the presen of the NpAg. The evaluation of the antimicrobial activity of the NpAg obtained against: Escherichia coli(ATCC 11229) , Staphylococcus aureus (ATCC 6538) , Chaetomium globosum (KU936228) and Alternaria alternata (KU936229) were performed using the agar diffusion technique (Kirby-Bauer).The NpAg obtained from the AgNO3 lowest concentration presented spectra with bands of absorption with maxima ~ 450 nm which confirm the presence of NpAg. The bands differ in width which is related to a wider particle size distribution, being NpAg from Ilex paraguariensis the widest and highest absortion intensity. The diffusion test showed that most of the Nps were active against fungi and bacteria.