Adjuvant effects of sulfolipo-cyclodextrin in a squalane-in-water and water-in-mineral oil emulsions for BHV-1 vaccines in cattle

ROMERA; HILGERS; PUNTEL; ZAMORANO; ALCON; DUS SANTOS; BLANCO VIERA; BORCA; SADIR

VACCINE

ELSEVIER SCI LTD

Año: 2000 p. 132 - 141

0264-410X

The antibody and cell mediated immune responses induced by BHV-1 were analysed in cattle after vaccination and challengeexposure to the virulent strain LA of BHV-1. Animals were vaccinated intramuscularly (IM) with inactivated virus vaccinesagainst BHV-1 containing either a water in mineral oil adjuvant (W/O), a water in mineral oil adjuvant plus Avridine (W/O+Avridine) or sulfolipo-cyclodextrin in squalane in-water emulsion (SL-CD/S/W). No signi®cant di€erences were registered inthe antibody response induced by the three evaluated vaccines. However, the BHV-1 speci®c cell-mediated immunite responsewas stronger and appeared earlier when SL-CD/S/W was included in the formulation. The ecacy of the vaccines was alsoevaluated after intranasal challenge of the calves with a virulent BHV-1 LA strain. Animals vaccinated with SL-CD/S/W hadreduced virus excretion and clinical symptoms compared with the mock-vaccinated animals. Comparison of levels of BHV-1specific IgG2 and IgG1 with virus shedding revealed that, regardless of the adjuvant administered, animals showing BHV-1speci®c IgG2/IgG1 ratios higher than 1 were those with a signi®cant lower number of individuals shedding virus. Additionally,animals vaccinated with SL-CD/S/W presented no post-vaccinal reactions. These factors, combined with the higher ecacy andthe ease of manipulation of the biodegradable oil, makes the vaccine formulated with this new adjuvant an importantcontribution for the veterinary vaccines industry.