Retinoic acid reduces migration of human breast and cervical cancer cells.

MARTINEZ MARIA LAURA; VANDERHOEVEN FIORELLA; REDONDO ANALIA; VARGAS-ROIG LAURA MARIA; SANCHEZ ANGEL MATIAS; FLAMINI MARINA INES

Mendoza

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2016

Sociedad de Biología de Cuyo

RETINOIC ACID REDUCES MIGRATION OF HUMAN BREAST AND CERVICAL CANCER CELLSMartinez AL, Vanderhoeven F, Redondo AL, Vargas Roig LM, Sánchez AM and Flamini MI. Laboratorio de Biología Tumoral. IMBECU-CCT-CONICET-Mendoza, Argentina. E-mail: mflamini@mendoza-conicet.gov.arBreast and Cervical cancer are common malignant diseases worldwide. Retinoids, are currently being used in clinical trials to treat or prevent cancer progression. We have previously demonstrated that long retinoic acid (RA) treatment (10-6M) reduced cellular adhesion and migration in breast cancer cells. In addition, we verified FAK/Paxillin and HSP27 nuclear translocation during 3-6 hours of RA treatment. As a consequence, we speculate that RA reduces breast cancer cell adhesion by inhibiting the formation of focal adhesion complexes and we hypothesize that FAK is carried by HSP27. Objectives: 1) To verify in T47D breast cancer cells if HSP27 interacts with FAK/Paxillin. 2) To determine in HeLa cervical cancer cells if FAK/Paxillin are translocated to the nucleus after RA treatment. 3) To define if RA decreases the migration of HeLa cells. Methodology: HeLa 1.3 (with HSP27 expression), HeLa 2.2 (without HSP27 expression) cervical cancer cells, T47D breast cancer cells, immunofluorescence, immunoprecipitation, western blot and migration assays. Results: We observed that FAK interacts with HSP27 in T47D cells and this interaction is increased after RA treatment. FAK translocates to the nucleus in HeLa 2.2 after RA treatment, suggesting that HSP27 is not essential in this process. Furthermore, RA treatment (10-5 M) decreases Hela 2.2 cells migration. Conclusion: HSP27 has not a crucial role in the inhibition of cancer cells migration and FAK nuclear translocation induces by RA.Topic: Tumoral and Molecular Biology. Province of origin: Mendoza, Argentina.