Reunión Anual de Sociedades de Biociencias 2023

MASCI, IVANA; LEZÓN, CHRISTIAN; DUGOUR ANDREA; BONETTO, JULIAN; FIGUEROA, JUAN MANUEL; TASAT, DÉBORAH; KURTZ, MELISA

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Inadequate nutrition and air pollution (gases and particulate matter-PM) are two majornongenetic environmental factors known to cause serious public health problemsworldwide. The nutritional status may modify the susceptibility to PM exposure andcause a wide range of acute and chronic diseases. PM depending on the concentration,composition and time of exposure may, through the release of pro-inflammatory andpro-oxidant mediators, exert its adverse impact on the individual?s health. The aim ofthis study was to evaluate in a nutritional growth retardation (NGR) animal model, thesubchronic exposure of Residual Oil Fly Ash (ROFA, an ambient air PM surrogate) onlung oxidative metabolism. Furthermore, we analyze in vitro the alveolar macrophage(AM) response to virus-like particles. Wistar male weanling rats were divided at randomin two groups. NGR animals, were fed during 4 weeks a restricted diet 20% comparedto ad libitum intake of Control (C) animals. NGR and C rats were intranasally instilledwith either 0.17mg/kg BW of ROFA or its vehicle 3 times a week during 1 month. Lungwas isolated and the oxidative metabolism was assessed by means of antioxidantenzymes activity (Catalase-CAT and Superoxide dismutase-SOD). AM obtained bybronchoalveolar lavage were isolated and cultured. After 24h AM cultures were exposedto 50 µg/ml PolyIC virus-like particle and cell viability was assayed. In lung, only asignificative CAT response to ROFA instillation was observed in C animals. AlthoughNGR animals displayed higher levels of both antioxidant enzymes, no changes wereobserved despite ROFA exposure. In vitro, only AM from NGR animals exposed toROFA and treated with PolyIC showed a significantly decrease on cell viability. Inconclusion: exploring lung antioxidant response and alveolar macrophage (AM)functionality offers valuable insights into the synergistic alterations of pulmonaryresponse to both relevant environmental stressors: air pollution and chronicundernutrition.