Oxidative stress imbalance after particulate ambient air pollution exposure in a chronic nutritional stress model

KURTZ, MELISA; ASTORT, FRANCISCO; LEZÓN, CHRISTIAN; CHAMPIN, GRACIELA; FERRARO, SEBASTÍAN; FRIEDMAN, SILVIA; TASAT, DÉBORAH; BOYER, PATRICIA

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016

Air pollution does not affect everyone equally. Therefore, we sought to study in growing animals under chronic nutritional stress (CNS), the oxidative metabolism after acute exposure to Residual Oil Fly Ash-ROFA, a known surrogate ambient air particle pollution. Twenty-eight weanling male Wistar rats were randomized in two groups regarding food intake: 1) ad libitum food intake (Control-C), or 2) 80% of the amount of food consumed by control (CNS). After 4 weeks animals were intranasally instilled either with ROFA (1mg/kg BW) or saline solution defining 4 groups: C, CNS, C+ROFA, CNS+ROFA. After 24h, total cell number (TCN), differential cell count (DCC) and superoxide anion (O2.-) generation were examined in broncheoalveolar lavage; superoxide dismutase (SOD) and catalase (CAT) antioxidant enzymes activity, in lung homogenates; and number of white cells (BWC)/mm3 was determined in blood. TCN augmented after ROFA exposure being only significant for C+ROFA group (C:6.9±1.5 vs C+ROFA:17.0±3.1. p