PPAR agonists are negative regulators of oxidative and nitrosative stress in fetuses from diabetic rats.

CAPOBIANCO, EVANGELINA; MARTÍNEZ, NORA; KURTZ, MELISA; JAWERBAUM, ALICIA

Santiago de Chile, Chile

Congreso; 6th World Congress on developmental origins of health and disease (DOHAD); 2009

Sociedad DOHAD

The intra-uterine milieu of a diabetic mother leads to alterations in fetal development and growth, with a consequent diabetogenic tendency in the offspring´s adulthood. Oxidative and nitrosative stress have been involved in the etiology of feto-placental anomalies. The peroxisome proliferator activated receptors (PPARs) are ligand activated nuclear receptors that regulate several inflammatory and developmental processes. Objectiveof this work was to evaluate the in vitro and in vivo putative effects of PPAR agonists as regulators of oxidative and nitrosative stress in fetuses from control and diabetic rats. Methods: from day 0.5 of pregnancy,control and streptozotocin-induced diabetic rats were fed with a standarddiet or with a standard diet supplemented with 6% olive oil (OO) or 6% safflower oil (SO), respectively containing 75% oleic acid and 75% linoleic acid, natural ligands of PPARs. Fetuses were explanted on day 13.5 of gestation. Fetuses from rats fed with the standard diet were cultured for 3 hours in the presence or absence of leukotriene B4(LTB4, 0.1uM), carbaprostacyclin (cPGI2, 1