AIR PARTICULATE MATTER INDUCES VASCULATURE DYSFUNCTION IN A NUTRITIONAL GROWTH RETARDATION RAT MODEL.

KURTZ, MELISA L; DEHEZA ZAHIRA; LEZÓN, CHRISTIAN; MASCI, IVANA; BOYER, PATRICIA; ALVAREZ, LAURA; TASAT, DÉBORAH

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021 LXVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2021

Around 2 billon children (almost 90% of the world´s child population) are exposed to air pollution levels over 10 µg/m3. Furthermore, child malnutrition is recognized as a major problem with devastating effects on children´s health. Due to their physical and physiological characteristics, children conform a subpopulation highly susceptible to the adverse effect of environmental pollutants (gases and particulate matter -PM) and vulnerable to malnutrition. PM exposure increases the risk for cardiovascular disease progression, through vascular oxidative stress and inflammation, two main processes involved in the initiation of endothelial dysfunction.Therefore, we investigate the effects of ROFA (Residual Oil Fly Ash) exposure on the vasculature, in a nutritional retardation (NGR) rat model. In order to achieve NGR animals, male weanling rats were fed a diet restricted 20% compared to ad libitum intake (control-C) for 4 weeks. NGR and C rats were intranasally instilled with either 1mg/kg BW of ROFA or its vehicle. 24h after exposure, the thoracic aorta was isolated and biochemical parameters were evaluated: CYP1A1, eNOS and Calcium channel type L by RT-PCR, TGFβ1 by Westen blot and cytokines (IL-6, IL-10) by ELISA. Our data showed that ROFA exposure induced IL-6 and IL-10 augmentation vs. C rats (35% and 30%, p