Identification and classification of Carya's introgressed material using SSR markers for its traceability in the Argentine productive system

GARCIA, MN; FRUSO, EA.; AGUIRRE, NC; VILLALBA, PV; RIVAS, JG; ACUÑA, CV; MARTINEZ, MC; MARCUCCI POLTRI, SN

Estambúl

Congreso; XXX International Horticultural Congress; 2018

This study was undertaken to develop DNA marker profiles that could be used to distinguish among 27 pecan (Carya illinoiensis) cultivars introduced from the United States (Apache, Caddo, Colby, Curtis, Desirable, Elliott, Forkert, Giles, Gloria Grande, Green River, Hirschi, Hodge, Kanza, Kiowa, Lucas, Major, Maramec, Nacono, Oconee, Osage, Pawnee, Peruque, Riverside, Stuart, Sumner, Western, Wichita) and used in production in Argentina. Oligonucleotide markers for microsatellite analysis were selected from Grauke et al. 2003. Only one primer pair was monomorphic and excluded from the analysis.The number of alleles per locus ranged from one to eight, in total 42 alleles have found, seven were private alleles (1 Apache, 1 Caddo, 1 Colby, 1 Giles and 3 Nacono). The Polymorphic Information Content (PIC) values ranged from 0.12 and 0.80.Genetic similarities were calculated and a dendrogram (UPGMA) has been established. A classification analysis was carried out using the ?complete? clustering method run for genotype and phenotype (obtained from ARS-USDA Pecan Database) with the NbClust R package. There were obtained K=3 and K=2 for genotypic and phenotypic information respectively and the clusters conformation were similar (87.25%).The genotypic information was consistent with the phenotypic data and it allowed to distinguish all varieties except two pairs: Pawnee-Kiowa and Sumner-Oconee. Phenotypic data showed that the pair Oconne-Forkert was the most similar and on the other hand, Stuart-Curtis the most different. Genotypic data points out Wichita-Pawnee, Wichita-Kiowa and Lucas-Hirschi as the most distant pairs.With seven SSR markers we can unequivocally differentiate 23 of the 27 introgressed varieties, being a valuable tool for the traceability of the material.