Transfer simple sequence repeat (SSR) markers from Eucalyptus spp. to E. sideroxylon in a cost-effective multiplex format.

RIVAS, JUAN G.; PATHAUER, PABLO S.; AGUIRRE, NATALIA C.; VILLALBA, PAMELA V.; GARCIA, MARTÍN N.; PALAZZINI, DINO A.; ACUÑA, CINTIA V.; MARTINEZ, M. CAROLINA; HOPP, ESTEBAN H.; MARCUCCI POLTRI, SUSANA N.

Harbin

Conferencia; The 20th IUFRO Tree Biotech and the 2nd FTMB (Forest Tree Molecular Biology and Biotechnology) Conference; 2022

In Argentina, slow-growing forest native species satisfy the demand for natural long-lasting hardwood. This implies a sustainability problem for the native forest, leading their population size to a risk point. Eucalyptus sideroxylon (A.Cunn. ex Woolls), is ranked among the most durable species and performs well against cold and drought. According to Standards Australia's AS 5604 2005 (Timber - Natural durability ratings), it is considered Type 1 (very durable without treatment).An important step in promoting the multiplication and use of E. sideroxylon as a source of Type 1 wood in the Argentinean pampas, is to know the genetic variability of the populations present in the region. In this sense, molecular markers are useful tools to address this issue.Therefore, due to the lack of microsatellite markers (SSR) developed for this species, we carried out the transferability of 28 SSR (neutral and functional markers) developed in E. grandis and E. globulus using a multiplex format.As a result, four amplification mixtures containing between seven to five markers were able to successfully transfer 23 SSR (82%) with reproducible and reliable amplicon patterns. Twenty-one of them were polymorphic and two monomorphic (distributed in 10 of the 11 Eucalyptus chromosomes) in four samples, the rest of them did not show an amplification product.Thanks to the multiplex PCR format and the successful cross-transferability of the SSR, this development will allow rapid genotyping at a low cost. The knowledge of the genetic variability will boost the establishment of a breeding program for E. sideroxylon in Argentina.