SLPI modulates the expression of adhesion and histocompatibility molecules on monocytes and hela cells

AMIANO NICOLAS; GUERRIERI DIEGO; COSTA MARIA JULIETA; MAFFIA PAULO; CHULUYAN EDUARDO

Córdoba, Argentina.

Congreso; VII Latinamerican Congress of Immunology; 2005

ALAI

The aim of this work was to study the ability of Secretory Leucocyte Protease Inhibitor (SLPI) to modulate the expression of surface molecules on monocytes and HeLa cells. The expression of CD54 (ICAM-1), MHC class I and MHC class II were analyzed with flow cytometry. The treatment of monocytes with rhSLPI (4mg/ml, 16h, 37°C) decreased the expression of CD54 (22%) and MHC II (9%), but not MHC I. The expression of CD54 was also decreased (30%) on HeLa cell line after the treatment. Furthermore, unlike for monocytes, the expression of MHC I was also decreased (40%) on HeLa cells. Since, HeLa produced significant amount of SLPI, we examined whether endogenous SLPI was able to modulate the expression of MHC I and CD54. By transfected HeLa with SLPI antisense oligonucleotides, the production of SLPI was reduced by 16% and the expression of MHC I increased by 27%. To improve the downmodulation of SLPI, the gene of SLPI was cloned with antisense orientation into mammalian expression vector (pcDNA3). Following, HeLa cells were transfected with this construction. Similar to the results obtained with antisense oligonucleotide, the expression of SLPI on the stable transfected cell line, was lower than non transfected cells and the expression of MHC I increased significantly (30%). Overall, these results suggest that endogenous and exogenous SLPI can modulate the expression of cell surface molecules.