Human Endothelial Cell Seeding in Partially Decellularized Kidneys

HAEUBLEIN, GERALDINE; LOMBARDI G; CARO FIORELLA; GUERRIERI DIEGO; REMOLINS CARLA; INCARDONA CLAUDIO; CASADEI DOMINGO; CHULUYAN EDUARDO

Biomed Research Internationa

Hindawi

Año: 2022

2314-6133

The excessive demand for organ transplants has promoted the development of strategies that increase the supply of immunecompatible organs, such as xenotransplantation of genetically modified pig organs and the generation of bioartificial organs.We describe a method for the partial replacement of rat endothelial cells for human endothelial cells in a rat’s kidney,obtaining as a final result a rat-human bioartificial kidney. First, in order to maintain parenchymal epithelial cells andselectively eliminate rat endothelial cells, three methods were evaluated in which different solutions were perfused through therenal artery: 0.1% sodium dodecyl sulfate (SDS), 0.01% SDS, and hyperosmolar solutions of sucrose. Then, partiallydecellularized kidneys were recellularized with human endothelial cells and finally transplanted in an anesthetized rat. Thesolution of 0.1% SDS achieved the highest vascular decellularization but with high degree of damage in the parenchyma side.On the contrary, 0.01% SDS and hyperosmolar solutions achieved a partial degree of endothelial decellularization. TUNELassays reveal that hyperosmolar solutions maintained a better epithelial cell viability contrasting with 0.01% SDS. Partiallydecellularized kidneys were then recellularized with human endothelial cells. Histological analysis showed endothelial cellsattached in almost all the vascular bed. Recellularized kidney was transplanted in an anesthetized rat. After surgery,recellularized kidney achieved complete perfusion, and urine was produced for at least 90 min posttransplant. Histologicalanalysis showed endothelial cells attached in almost all the vascular bed. Therefore, endothelial decellularization of grafts andrecellularization with human endothelial cells derived from transplant recipients can be a feasible method with the aim toreduce the damage of the grafts.