Immunoprotection against T. cruzi infection elicited by a multicomponent vaccine

CAZORLA SILVIA INÉS; MATOS MARINA NADIA; RAMIREZ CAROLINA VERÓNICA; SANCHEZ ALBERTI ANDRES; MALCHIODI EMILIO LUIS

Congreso; 1st French-Argentine Immunology Congress. LVIII Reunión Anual de la Sociedad Argentina de Inmunología.; 2010

We have previously demonstrated that Salmonella enterica as DNA delivery system for the major cystein proteinase of T. cruzi, cruzipain (SCz), is able to induce immunoprotection against Chagas disease. To improve the performance of this DNA vaccine, we tested the co-administration of SCz and Salmonella (S) carrying genes encoding Tc52 (a thiol-transferase) and Tc24 (a 24 kDa flagellar calcium binding protein). Mice were immunized with: GI- PBS (Control), GII: SCz, GIII: STc52; GIV: STc24; GV: SCz+STc24+STc52. We found that only antibodies generated in GV vaccinated mice were able to increase the complement-mediated killing of T. cruzi (p