INVESTIGADORES
LARA Ruben Jose
artículos
Título:
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments.
Autor/es:
PRIOTTO, S.; LARA, R.J.
Revista:
Journal of Marine Biology & Oceanography
Editorial:
SciTecnhol Publisher
Referencias:
Lugar: Foster City; Año: 2013 p. 1 - 5
ISSN:
2324-8661
Resumen:
In the present work different approaches
were tested for the optimization of the hydrolysis conditions for the joint
determination of amino acids and amino sugars in chitin, an important
structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton.
Ten treatments were evaluated, involving different combinations of hydrolysis
temperature and duration. The obtained results indicate that slightly lower
temperatures (105ºC) than for the classical combined amino acid hydrolysis and
longer times (22 hours) than for chitin minimized losses and maximized
recoveries of monomeric amino acids and amino sugars. This represents a large
advantage in comparison to the usual methods of separate hydrolysis for
high-performance liquid chromatography (HPLC) determination, allowing a
simultaneous analysis of both compound classes in a single run.