Oxidative stress modulates GSK3β signaling associated with lipolysis in fat cells.

FUNK, M.I.; MANISCALCHI, A.; BENZI JUNCOS, O.N.; ALZA, N.P.; CONDE, M.; SALVADOR, G.; URANGA, R.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2023; 2023

Oxidative stress (OS) modulates fat metabolism and triggers chronic inflammation, promoting the onset of metabolic diseases such as type 2 diabetes and obesity. We have previously demonstrated that iron-induced OS increases lipolysis in mouse white adipose tissueas well as in in vitro differentiated adipocytes. We found that iron treatment triggered β-catenin upregulation and exacerbated lipolysis by ATGL activation. In addition, adipocytes where β-catenin had been deleted showed no ATGL upregulation by OS. Our aim was to study the role of GSK3β kinase in the modulation of β-catenin cascade in adipocytes exposed to OS. For this purpose, we used differentiated 3T3-L1 adipocytes (0.5 mM IBMX, 1 μM dexamethasone, 6 μg/ml insulin, and 5 μM rosiglitazone) exposed to 500 μM ferric ammonium citrate (FAC) for 24 h. We also analyzed adipose tissue and liver isolated from mice challenged with iron overload. As previously reported in adipose tissue, OS enhanced lipolysis in liver (p