INVESTIGADORES
ZAPPACOSTA Diego Carlos
artículos
Título:
Changes in isoperoxidases patterns during the in vitro rooting of Nothofagus antarctica
Autor/es:
MARTINEZ PASTUR G; ZAPPACOSTA D; ARENA M; CURVETTO N
Revista:
Bulgarian Journal of Plant Physiology
Editorial:
Institute of Plant Physiology
Referencias:
Lugar: Sofía, Bulgaria; Año: 2001 vol. 27 p. 43 - 53
Resumen:
Peroxidases play a major role in several physiological processes..
Specifically, in vitro activity of this enzyme was used as a marker to identify
different rooting phases of Nothofagus. The study of peroxidase profile patterns
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
different rooting phases of Nothofagus. The study of peroxidase profile patterns
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
in vitro activity of this enzyme was used as a marker to identify
different rooting phases of Nothofagus. The study of peroxidase profile patterns
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
Nothofagus. The study of peroxidase profile patterns
during rhizogenesis in this genus could give additional information
about this topic, to justify the use of peroxidase isoforms as a more specific
biochemical marker than total peroxidase activity. This work gives information
on qualitative and quantitative changes in isoperoxidase expression
(basic and acidic peroxidases) during in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
in vitro rooting of N. antarctica microshoots
through native PAGE. Time course analysis of isoperoxidase activity
during rhizogenesis allowed positive correlation with specific morphogenic
stages. Seventeen peroxidase bands were detected, 4 basic and 13 acidic. Four
of the acidic peroxidases appeared during the induction stage of rooting. Variation
in the number and relative activity of the isoperoxidases was found during
different stages of adventitious rooting. The variation in the number and
activity of isoperoxidases provides a good indicator of the processes involved
in adventitious rooting of N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.
N. antarctica. Possible linkage of basic isoperoxidases
with auxin metabolism and the role of certain isoperoxidases in specific
rooting processes are also discussed.