INVESTIGADORES
LAVALLEN Carla Mariela
artículos
Título:
First morphological and molecular analysis of Eucoleus boehmi like eggs in dogs from Argentina
Autor/es:
CARLA MARIELA LAVALLÉN; ROMINA SANDRA PETRIGH; MARTÍN HORACIO FUGASSA; GUILLERMO MARÍA DENEGRI; MARCELA CECILIA DOPCHIZ
Revista:
PARASITOLOGY RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2018 vol. 117 p. 2351 - 2357
ISSN:
0932-0113
Resumen:
The canid parasites Eucoleus aerophilus (syn. Capillaria aerophila) and Eucoleus boehmi (syn. Capillaria boehmi) parasitize the lower and the upper respiratory tract respectively. Reports and descriptions of these nematodes are scarce in Argentina, possibly due to misdiagnosis of morphologically similar trichuroids eggs, and the lack of knowledge about the species of Eucoleus in this geographical area. Scanning electron microscopy is a useful tool for identification of E. boehmi eggs based on the characteristics of the shell structure which differentiate between species. Molecular analysis complements morphological identification. Until now, there are no studies based on the analysis of E. boehmi eggs in Argentina. The aim of the present work was to study by morphological, morphometric and molecular analysis, eggs attributable to E. boehmi isolated from dogs naturally infected in Mar del Plata city, Argentina. Eggs isolated from two dog fecal samples were analyzed by light and scanning electron microscopy. A fragment of mitochondrial DNA (mtDNA) of the cytochrome c oxidase subunit I gene (cox1) from eggs was sequenced, and phylogenetic analysis was performed in this study. According to morphological results based on the wall surface ultrastructure the eggs studied were assigned to E. boehmi. Molecular analysis supported the morphological identification. The divergence of 9-12% with the European isolated could suggest a new geographical genetic variation of E. boehmi, but also question the possible existence of cryptic species. This is the first characterization of E. boehmi eggs in dogs from Argentina.