Prebiotics improved growth and hypocholesterolemic effect of the probiotic strain Limosilactobacillus reuteri CRL 1098

GOMEZ, JORGE; LEDESMA, ANA E.; TARANTO, MARÍA PÍA; BUSTOS, ANA YANINA

Encuentro; LI Reunion Anual de la sociedad Argentina de Biofisica; 2023

The field of cardiovascular health management now emphasizes the potential benefits ofsynbiotic combinations comprising both probiotics and prebiotics. Limosilactobacillus (L.)reuteri CRL1098 is a probiotic bacterium known for its hypocholesterolemic effects linkedto its bile salt hydrolase (BSH) enzyme. The purpose of the present study was toinvestigate the potential benefit of different prebiotics on bacterial growth, BSH activityand bile acid tolerance of CRL 1098 strain for the design of a synbiotic withhypocholesterolemic effect.To achieve this goal, MRS medium was supplemented with different prebiotics, includinginulins with varying degrees of polymerization (FOS-P95, GR, HSI, and HP) and lactulose,at final concentrations ranging from 0.5% to 2.0% (w/v). Our results showed that theaddition of lactulose improved probiotic growth respect to glucose with relative growthrates in a range of 1.7 to 2.5, while inulins do not modify the growth of the strain.Remarkably, all prebiotics increased the BSH activity of the strain, thus enhancing itsprobiotic properties. The best results were observed with the addition of 2% whichincreased the activity by 20%, reaching almost 90% hydrolysis. However, prebiotics donot improve the survival of probiotics in the presence of bile acids. Our results showedthat lactulose was able to promote probiotic growth and also have benefits on BSH activitywhich may ultimately positively modulate cholesterol metabolism. However, ifdeconjugation of bile salts with a view to lowering serum cholesterol is the main objectiveof the synbiotic formulation, inulin-type prebiotics can be considered, as even at lowconcentrations all were able to enhance BSH activity.