INVESTIGADORES
STROBL MAZZULLA Pablo Hernan
congresos y reuniones científicas
Título:
Control Neural Crest Specifiers by Histone Demethylases JmjD2A
Autor/es:
STROBL MAZZULLA, P.H.; BRONNER-FRASER, M.
Reunión:
Congreso; Experimental Biology Meeting 2010; 2010
Resumen:
The neural crest (NC)
is a multipotent, migratory stem cell-like population that is able to
differentiate in many cell derivatives including the peripheral nervous system
and facial skeleton of vertebrate embryos. Many studies have been focused on
transcriptional regulation underlying NC formation, but less is known about
epigenetic influences. Accordingly, we find unexpectedly dynamic epigenetic
modifications are critical for proper temporal control of neural crest gene
expression in vivo. The histone
demethylase, JumonjiD2a (JmjD2A), is expressed in the forming neural folds and
its loss of function using morpholinos causes a dramatic down-regulation of
neural crest specifier genes. Importantly, chromatin immunoprecipitation
reveals stage-specific interactions of JmjD2A with regulatory regions of neural
crest genes as well as associated stage-specific modifications in the
methylation states of critical lysine residues. The results clearly show that
epigenetic events are important for regulating the chromatin structure on
neural crest genes, providing the first in
vivo evidence for an epigenetic modification directly controlling a
critical developmental event in the vertebrate embryo.