Progenitor Radial Cells and Neurogenesis in Pejerrey Fish Forebrain

STROBL MAZZULLA, P.H.; NÚÑEZ, A.; PELLEGRINI, E.; GUEGUEN, M.; KAH, O.; SOMOZA, G.M.

BRAIN BEHAVIOR AND EVOLUTION

KARGER

Año: 2010 vol. 76 p. 20 - 31

0006-8977

The central nervous system of adult teleost fish is peculiarbecause of the following features: (1) the persistence of radialglial cells, (2) an important neurogenic activity and (3) ahigh aromatase expression by radial cells. In this study, theproliferative zones of the forebrain were described usingbromodeoxyuridine (BrdU) treatment in the brain of thepejerrey, an Acanthopterygian teleost fish. These cells wereshown to have morphological and immunoreactive characteristicsof radial cells and to express aromatase. Three differentprogenitor populations were identified based on themobility and proliferation capacity 6 weeks after BrdU treatment:transit amplifying progenitors, slowly proliferatingstem cells, and cells remaining in the proliferative zonesshowing no signs of mitotic activity. The proliferative cellswere always located in the ventricular zone and were neverobserved in the brain parenchyma; however, 3 weeks laterthey were found away from these proliferative zones anddisplayed acetylated tubulin immunoreactivity. Other BrdUpositivecells showed astrocyte morphology and were im-munoreactive to the S100 glial marker. These results showthat in this fish, radial cells are true progenitors generatingneurons and possibly astrocytes.