congresos y reuniones científicas
Biogenesis of extracellular vesicles released by the parasite Trichomonas vaginalis
Congreso; XXXI Reunión Anual. Sociedad Argentina de Protozoología; 2019
T. vaginalis is a parasite that causes trichomoniasis, the most common non-viral sexually disease worldwide. Given it is an extracellular parasite, adhesion to host cells is one of the key processes for the development of infection. In recent years, various factors that influence this process have been identified, including extracellular vesicles (EVs). Previously in our laboratory we demonstrated that both exosomes (vesicles with a size range of 40-100 nm) and ectosomes (vesicles with a size of 100 - 1000 nm) are involved in the process of interaction of T. vaginalis with host cells. It is currently known that ESCRT-III subunit of to the ESCRT complex is involved in membrane cleavage being the VPS32 protein the key effector during membrane scission. These antecedents lead to our hypothesis that VPS32 might be regulating the process of extracellular vesicles formation in T. vaginalis. Based on this, we transfected the protein VPS32 and an empty vector as a control (EpNEO) in a poorly-adherent parasite strain and evaluated the amount of EVs released by these parasites by TEM, NTA and western blot using Evs markers. Our results demonstrated that VPS32 transfected parasites released more EVs than EpNEO parasites. The amount of both EVs populations is affected; suggesting that VPS32 is involved in the biogenesis of exosomes as well as ectosomes. As our previous results demonstrated that EVs are regulating parasites adhesion and now, we observe that VPS32 parasites produces more EVs, we performed an adhesion assay to host cells to evaluate the adherence capacity of VPS32 and EpNEO parasites. Interestingly, our results indicate that parasites transfected with VPS32 are ~40 times more adherents than EpNEO parasites. In summary, our results demonstrated that VPS32 is a key player in the regulation of the adherence process; provably due to its role in the increased biogenesis of extracellular vesicles.