TFVPS32 regula la división celular en el parasito Tritrichomonas foetus

IRIARTE, LUCRECIA S.; DE MIGUEL, NATALIA; COCERES, VERONICA M.

Santa Fe

Congreso; XXVIII Reunión Anual. Sociedad Argentina de Protozoología; 2016

SAP

The flagellated protist Tritrichomonas foetus is a parasite that causes bovinetrichomonosis, a major sexually transmitted disease in cattle. Cell division hasbeen described as a key player in controlling cell survival in other cells, includingparasites but there is no information on the regulation of this process inT. foetus. The regulation of cytokinetic abscission, the final stage of cell division,is mediated by members of the ESCRT (endosomal sorting complexrequired for transport) machinery. VPS32 is a subunit within the ESCRTIII complexand here, we report that TfVPS32 is localized on cytoplasmic vesicles anda redistribution of the protein to the midbody is observed during the cellulardivision. In concordance with its localization, deletion of TfVPS32 C-terminalalpha helices (a5 helix and/or a4-5 helix) leads to abnormal T. foetus growth,an increase in the percentage of multinucleated parasites and cell cycle arrestat G2/M phase. Together, these results indicate a role of this protein in controllingnormal cell division