DE MIGUEL Natalia
congresos y reuniones científicas
EPIGENETIC REGULATION OF GENES IMPLICATED IN ADHESION TO HOST CELLS IN TRICHOMONAS VAGINALIS
PACHANO, T; VILLARRUEL, F; LIZARRAGA, A; COCERES V.M; STROBL-MAZZULLA, P.H; DE MIGUEL N
Congreso; X Congreso Argentino de Protozoología y Enfermedades Parasitarias; 2014
Sociedad Argentina de Protozoologia
Trichomoniasis, caused by the flagellated protozoan Trichomonas vaginalis, is the most common non-viral sexually transmitted infection. Adhesion to the host epithelial cells is an essential step for the survival of this extracellular parasite and the process of pathogenesis. Surface proteins implicated in adhesion to mucosal epithelial cells were shown to be differentially expressed in pathogenic and non-pathogenic strains. Understanding the mechanisms involved in the control of genes related to pathogenesis and host cell adhesion could assist in the development of new therapeutic strategies. However, regulation of gene expression and the epigenetic influence in this parasite are poorly understood. To elucidate the existence of histone modification possibly implicated in the transcriptional regulation of genes associated with T. vaginalis pathogenesis, we performed an immunofluorescence assay using antibodies against histone epigenetic marks. Histone modifications associated with active (H3Kac and H3K4me3) and repressive (H3K9me3 and H3K27me3) chromatin states were found in the pathogenic strain B7268 and non-pathogenic strain G3. In vivo chromatin immunoprecipitation assays followed by qPCR on these parasite strains reveal an association between repressive and permissive histone modifications and the expression of genes implicated on the parasite attachment to human host cells.