Self-Assembled Multilayers of Water-Soluble Modified-Chitosan and Glucose Oxidase For Detection of Glucose Oxidase for Detection of Glucose in Millk Samples

FLORENCIA A. GULOTTA; LADISLAO DIAZ VERGARA; MARIANA MONTENEGRO; NANCY F. FERREYRA; VERÓNICA I. PAZ ZANINI

Conferencia; Southern Brazilian Journal of Chemistry International Virtual Conference; 2022

Multilayer films made of glucose-functionalized chitosan (G-Chit) and glucose oxidase (GOx) were built by layer-by-layer self-assembly technique onto carbon paste electrodes (CPE). The obtained bioelectrodes were characterized by cyclic voltammetry and chronoamperometry. Results indicated that catalytic response increases with the number of bilayers G-Chit/GOx and the enzyme concentration obtaining the best responses for 3 bilayers and 2 mg ml-1, respectively. The effect of pH on the bioelectrode response was also investigated, it was found that the optimal working value is 7.0. Under optimized experimental conditions, the biosensor exhibits a sensitivity of (0.81 ± 0.03) µA mM-1 in a glucose concentration range 0.18 to 1.75 mM. The analytical response of the biosensor was tested in milk samples with negligible matrix effects. Results show that G-Chti appears promising for the immobilization of enzymes.