Photochemistry of 6-formylpterin in alkaline medium

ANDRÉS H. THOMAS; GUSTAVO SUÁREZ; FRANCO M. CABRERIZO; ALBERTO L. CAPPARELLI

HELVETICA CHIMICA ACTA

Wiley_VHC

Año: 2001 p. 3849 - 3860

0018-019X

6-formylpterin solutions at pH 11 were photolyzed at 350 nm at room temperature. The photochemical reactions were followed by UV/VIS spectrophotometry, thin layer chromatography (TLC), and highperformance liquid chromatography (HPLC). In the presence of oxygen, 6-carboxypterin is the only photoproduct detected by the analytical techniques mentioned. In the absence of oxygen, a new compound showing an absorbance maximum at 480 nm is observed. The latter compound is thermally oxidized very fast in the presence of oxygen to 6-carboxypterin. The quantum yields of substrate disappearance and of photoproduct formation are reported.