Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions

LUCIANA ISAJA; LUJÁN FERRIOL-LAFFOUILLERE, SOFÍA; SOFIA MUCCI; MARÍA SOLEDAD RODRÍGUEZ-VARELA; LEONARDO ROMORINI

Methods in Molecular Biology

Springer

Año: 2021;

Human embryonic stem cells (hESCs) can differentiate into any cell lineage (pluripotency potential) derived from the three germ layers: ectoderm, mesoderm and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we describe a EBs-based protocol that allows multilineage differentiation of hESCs and human induced pluripotent stem cells (hiPSCs) grown on feeder-free conditions.