INVESTIGADORES
LLARRULL Leticia Irene
congresos y reuniones científicas
Título:
Overexpression of the BlaR1 membrane protein implicated in resistance to beta-lactams in Staphylococcus aureus
Autor/es:
BELLUZO,B.S.; LLARRULL, L.I.
Lugar:
Villa Carlos Paz
Reunión:
Congreso; XLII Reunion Anual de la Sociedad Argentina de Biofisica; 2013
Institución organizadora:
Sociedad Argentina de Biofisica
Resumen:
BlaR1 is a membrane
receptor implicated in beta-lactam antibiotic resistance in Staphylococcus aureus. This protein is composed of various
transmembrane helixes, followed by a cytoplasmic domain and an extracellular
sensor domain capable of detecting de presence of antibiotics (1). The predicted
cytoplasmic domain contains Zn(II)-dependent protease activity, and it has been
proposed that it is activated in response to binding of the antibiotic to the
sensor domain (2). Activated BlaR1 degrades the gene repressor BlaI, which
triggers the expression of the BlaZ beta-lactamase. We are interested in the
spectroscopic study of the signal transduction mediated by BlaR1. In order to
overexpress this protein, we used active natural variants lacking the first
three transmembrane helixes. We evaluated the expression as fusions to
different soluble tags that were shown to help in the overexpression of several
membrane proteins, and we are now developing a protocol of purification from
inclusion bodies, denaturation and refolding.
Acknowledgements
The PEW Charitable
Trusts, ANPCyT, CONICET
References
H.
Z. Zhang, C. J. Hackbarth, K. M. Chansky, H. F. Chambers, Science 291, 1962
(2001)
L.
I. Llarrull, S. Mobashery, Biochemistry 51, 4642 (2012).