Characterization of DNA Layers Adsorbed on Glassy Carbon Electrodes

MARÍA L. PEDANO, LÍA I. PIETRASANTA, MANUEL LÓPEZ TEIJELO, GUSTAVO A. RIVAS.

ELECTROANALYSIS

Willey Interscience

Año: 2008 vol. 20 p. 739 - 749

1040-0397

A DNA layer adsorbed at glassy carbon electrodes (GCE) was characterized by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of the adsorbed DNA layer on polished glassy carbon electrodes was assessed indirectly by ellipsometric measurements. Ellipsometry was also useful to evaluate the influence of the oxide layer formed on glassy carbon electrodes, either spontaneously or after electrochemical pretreatments, on the DNA adsorption and further electrooxidation process. SEM and AFM images of the electrode surface covered by a thick layer of DNA reveal a nonuniform distribution, leaving channels and islands of the biological material.