INVESTIGADORES
ACEVEDO Diego Fernando
congresos y reuniones científicas
Título:
Design of a New Electrogenerated Polyquinone Film Substituted with Oligopeptide Glutathione : Towards a Direct Electrochemical DNA Sensor
Autor/es:
ACEVEDO D.; KOROWITCH, A.; PIRO, B.; NOEL, V.; BUCHET, I.; BARBERO, C.A.; PHAM, M.C; , REISBERG, S.
Lugar:
los cocos. cordoba
Reunión:
Simposio; archipol 09; 2009
Institución organizadora:
archipol
Resumen:
Wedeveloped a method to graft a tripeptide (glutathione) onto 5-hydroxy-1,4-naphthoquinone, an electropolymerizable molecule. The resulting thin conducting polymer presents a well-defined and stable electroactivity in neutral buffered solution, due to the embedded quinone group, and is able to covalently graft amino-modified DNA probe strands. It is shown that the bioelectrode presents positive current change following DNA hybridization. This makes a signal-on direct electrochemical DNA sensor. The results were obtained with low target concentration (50 nM) and the selectivity is excellent as a single-mismatch sequence can be discriminated from the full-complementary target.
