ENHANCING THE REUSABILITY OF ENZYMES IN BIOETHANOL PROCESS BY POLYACRILAMIDE GRAPHENE OXIDE COMPOSITES

MULKO; J. PEREYRA; CUELLO, EMMA; C. R. RIVAROLA,; ACEVEDO D.; BARBERO C

cordoba

Simposio; XII SIMPOSIO ARGENTINO DE POLÍMEROS; 2017

unc

immobilization techniques that preserve and enhance the activity of biomolecules have huge potential intechnological applications. Several materials as immobilization matrices have been implemented in the recentpast to improve traditional enzymes performance in order to enhance the activity and the storage stability.[1,2]. These parameters are essential to promote the enzyme biocatalyst reusability and to decrease thecommodity cost generated in industrial biotechnology processes. In this work alpha-amylase enzymes areimmobilized into 3D acrylamide network polymer (PAAm) and polyacrylamide-graphene oxide composite(PAAm-GO). The biocatalyst supported materials (PAAm and PAAm-GO) were characterized by SEMmicroscopy, swelling and mechanical properties were measured. Moreover, the encapsulation capability andthe catalytic efficiency of these new supported biocatalysts were studied [3,4]. The results show that thePAAm support improves the biocatalyst reuse in comparison with the free enzyme and with the entrappedenzyme in a classical support as alginate. Moreover, it has been demonstrated that the mechanical properties,the encapsulation capability and the reusability of the enzyme are greatly improve using PAAm-GO compositeas scaffold. All the data support the importance of generate a protective environment to the biocatalyst inorder to increase the bioethanol productivity and reusability of the system.