INVESTIGADORES
KAMENETZKY Laura
artículos
Título:
High-throughput characterization of Echinococcus spp. metacestode miRNomes
Autor/es:
CUCHER, M.; MACCHIAROLI, N.; KAMENETZKY, L.; MALDONADO, L.; BREHM, K.; ROSENZVIT, M. C.
Revista:
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 45 p. 253 - 267
ISSN:
0020-7519
Resumen:
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Abstract: Echinococcosis is a worldwide zoonosis of great public
health concern, considered a neglected disease by the WHO. The
cestode parasites Echinococcus granulosus sensu lato (s. l.) and
Echinococcus multilocularis are the main aetiological agents. In the
intermediate host, these parasites display particular developmental
traits that lead to different patterns of disease progression. In an
attempt to understand the causes of these differences, we focused on
the analysis of microRNAs (miRNAs), small non-coding regulatory RNAs
with major roles in development of animals and plants. In this work,
we analyzed the small RNAs expression pattern of the metacestode, the
stage of sanitary relevance, and provide a detailed description of
Echinococcus miRNAs. Using high-throughput small RNA sequencing, we
have carried out the first experimental identification of miRNAs in
E. multilocularis and have expanded the Echinococcus miRNA catalog to
38 miRNA genes, including one miRNA only present in E. granulosus s.
l. Our findings show that although both species share the top 5
highest expressed miRNAs, thirteen are differentially expressed which
could be related to developmental differences. We also provide
evidence that uridylation is the main miRNA processing mechanism in
Echinococcus spp. Our results provide detailed information on
Echinococcus miRNAs which is the first step to understand their role
on parasite biology and disease establishment and/or progression and
their future potential use as drug or diagnosis targets.