Differentially methylated DNA sequences from tomato reciprocal hybrids

GIMENEZ, MAGALI DIANA; CAMBIASO, VLADIMIR; RODRÍGUEZ, GUSTAVO RUBÉN

Congreso; International Congress of Genetics 2018; 2018

Reciprocal hybrids from three tomato crosses hadshown differences in quantitative fruit traits. The parental genotypes of thosehybrids were Gema FCA and Querubín FCA, Caimanta and LA722 of Solanumpimpinellifolium and Red Purple and Zebra Green respectively. Additionally, itwas determined the occurrence of genomic imprinting between the reciprocalhybrids throughout MSAP (Methylation Sensitive Amplified Polymorphism) analysisat mature green stage fruits. This analysis revealed 10 epiloci differentially methylatedamong reciprocal hybrids and the highest number was detected in Gema FCA ?Querubin FCA cross. In order to know the DNA sequence in these epiloci, thebands were extracted from the acrylamide gels, amplified by PCR and sequenced.The sequences were analyzed using BLAST at the Sol Genomics Network database.The 10 epiloci belong to sequences present in chromosomes 3, 4, 6, 8, 10 and 12of the tomato genome. Six of them presented homology with coding regions ofIPT9 IPT2, At1g72480, ETO1, GMI1, DEGP2 and TRZ1 genes which code the proteinstRNA isopentenyltransferase, lung seven transmembrane receptor family,ethylene-overproduction protein, gamma-irradiation and mitomycin c induced 1,protease do-like 2 ? chloroplastic and nuclear ribonuclease Z respectively. Theremaining 4 epiloci correspond to non-coding regions, some of them contain theregulatory elements TATA-box and circadian of S. lycopersicum that could beaffecting the expression of nearby genes. These results indicate that DNAmethylation in coding and non-coding regions could trigger the observedphenotypic differences between reciprocal hybrids