TRANSCRIPTOMIC AT SEVERAL RIPENING STAGES IN TOMATO GENOTYPES (SOLANUM SPP.) DIFFERING FOR FRUIT SHELF LIFE

PEREIRA DA COSTA, JAVIER H; RODRÍGUEZ, GUSTAVO RUBÉN; PRATTA, GUILLERMO RAUL; PICARDI, LILIANA AMELIA; ZORZOLI, ROXANA

Foz do Iguazú

Congreso; 11th International Congress of Plant Molecular Biology; 2015

Shelf life is an important attribute for fresh market tomato. Fruit quality is determined by the fruit ripening stage at harvest time. The aims of this work were to study in two different experiments the differential expression of transcripts at three fruit ripening stages: Mature Green (MG), Breaker (B), and Red Ripe (RR) in the following genotypes: two cultivars of S. lycopersicum (Caimanta with normal ripening and the accession 804627 that carries mutant alleles at norgene) and two wild genotypes (LA1385 of S. lycopersicum var. cerasiforme and LA722 of S. pimpinellifoliumboth carrying unknown genes that prolong fruit shelf life[1,2]). In the second experiment fruits of same genotypes were compared in their expression profiles from B to RR when ripening in two different environments: plant or shelf. Standard protocols for RNA extraction and cDNA-AFLP were applied. Unique bands were detected for each ripening stage. A lower polymorphism was found in the transition from the stage MG to B than from stage B to RR in all genotypes except for the 804627. Nevertheless twenty differentially expressed transcripts, mostly involved in mechanism of stress responses, were found among all genotypes in the second experiment. We detected exclusive transcripts at each ripening stage and genes which expression is maintained during fruit ripening process. Moreover, we observed that Caimanta was the most polymorphic genotype according to the change of environmental conditions of ripening. References: 1Rodríguez et al. 2010 Euphytica176:137-147.2Pereira da Costa et al. 2013 ScientiaHorticulturae 156:47-53.