Preservation of Solanum pimpinellifolium genomic fragments in recombinant genotypes increase tomato fruit quality

MAHUAD, SABINA L; PRATTA, GUILLERMO RAUL; RODRÍGUEZ, GUSTAVO RUBÉN; ZORZOLI, ROXANA; PICARDI, LILIANA AMELIA

JOURNAL OF GENETICS

INDIAN ACAD SCIENCES

Lugar: Bangalore ; Año: 2013 vol. 92 p. 195 - 203

0022-1333

Five Recombinant Inbred Lines (RILs) obtained from the F2 generation of an interspecific cross between cultivar Caimanta (Cai, S. lycopersicum) and wild accession LA722 (P, Solanum pimpinellifolium) were crossed to obtain the Second Cycle Hybrids (SCH). Eleven fruit quality traits were assessed in evaluating phenotypic variability among genotypes P, Cai, F1(CaixP), five RILs, and ten SCH. One of the RILs and three SCH had higher values than P as the best genotype for shelf life. Sequence-Related Amplified Polymorphism (SRAP) was used as molecular markers for detection of polymorphism among these 18 genotypes. The percentage of polymorphism in RILs and SCH was 61% and 66% respectively. Moreover, some bands detected in P were present in SCH. Several multivariate analyses were performed to find agreement between the phenotypic variability observed for fruit quality traits and the polymorphism obtained from SRAP markers. A General Procrustes Analysis estimated that there was a consensus proportion of 75% between phenotypic and molecular data. There was considerable preservation of some bands from the wild genotype, which could increase the variability in fruit quality traits in populations where the genetic diversity is limited.