Bordetella holmesii in children suspected of pertussis

BOTTERO DANIELA; GRIFFITH, MATTHEW ; LARA, CLAUDIA; FLORES, DARÍO; PIANCIOLA, LUIS; GAILLARD M. EMILIA; FIORI, SILVANA; GALAS M.; TONDELLA, MARÍA LUCÍA; DANIELA HOZBOR

EPIDEMIOLOGY AND INFECTION.

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2012 vol. 5 p. 1 - 4

0950-2688

Bordetella pertussis causes an upper respiratory infection in infants, adolescents, and adults. Diagnosis of pertussis, a vaccine-preventable disease, can be difficult, but recent implementation of real-time PCR assays in laboratories has hastened the ability of clinicians to make an accurate diagnosis. In this paper we describe the method of nasopharyngeal specimen collection, extraction of DNA, and real-time PCR assays that will allow the detection and identification of Bordetella spp. in clinical specimens