INVESTIGADORES
RAYA Raul Ricardo
artículos
Título:
Sequence analysis of pLBB1, a cryptic plasmid from Lactobacillus delbrueckii subsp. bulgaricus.
Autor/es:
AZCÁRATE-PERIL MA, RAYA RR.
Revista:
CANADIAN JOURNAL OF MICROBIOLOGY
Editorial:
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
Referencias:
Lugar: Ottawa, ON K1A 0R6 Canada; Año: 2002 vol. 48 p. 105 - 112
ISSN:
0008-4166
Resumen:
The first report of the complete nucleotide sequence of a cryptic
plasmid from Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus
bulgaricus) is presented. The plasmid pLBB1 consists of 6127 bp with a
GC content of 44.8%. No ssDNA was detected by hybridization experiments,
which is consistent with the notion that pLBB1 does not replicate by a
rolling circle mechanism. A putative replication region of pLBB1 was
cloned and found to be functional in Lactobacillus johnsonii and
Lactococcus lactis. Plasmid pLBB1 showed significant DNA sequence
identity with plasmid pLL1212 from Lactobacillus delbrueckii subsp.
lactis (Lactobacillus lactis) CRL1212 (GenBank accession No. AF109691).
Four open reading frames (ORFs) larger than 100 amino acids were
identified. ORFA shared similarity with a putative primase-helicase
system, and ORFB and ORFC exhibited limited identity with a mobilization
protein and a transposase, respectively. Curing experiments did not
allowed us to assign a function to the ORFs.