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KATZ Eleonora
congresos y reuniones científicas
Título:
Gaba Fails to regulate the release of ACh at efferent-cochlear hair cell synapse in GABAB-knock out mice.
Autor/es:
CAROLINA WEDEMEYER; JAVIER ZORRILLA DE SAN MARTÍN; ANA VANESSA TORBIDONI; PAUL A. FUCHS; ANA BELÉN ELGOYHEN; ELEONORA KATZ
Lugar:
Huerta Grande, Córdoba, Argentina
Reunión:
Congreso; Segunda Reunión Conjunta de la Sociedad Argentina de Neurociencias (SAN) y el Taller Argentino de Neurociencias (TAN); 2010
Resumen:
During neonatal development
inner hair cells (IHCs) of the mammalian cochlea are transiently innervated by
medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) is the main
neurotransmitter released at this synapse but g-aminobutiric acid (GABA)
is also present at MOC synaptic terminals. We have previously shown by electrophysiological and pharmacological
methods that GABA modulates the
cholinergic input at MOC-IHC synapses by acting on presynaptic GABAB
receptors. To further evaluate the role of GABAB receptors in the modulation
of ACh release, we recorded postsynaptic currents evoked by electrically stimulating the MOC efferent
fibers contacting IHCs in acutely isolated mouse organs of Corti from GABAB-knockout
mice. Application of the specific GABAB agonist, baclofen (1mM), caused a significant reduction in the quantal
content of evoked release in wild-type
mice (37.3±6.1%
of control, n=4, p < 0.01) but did not affect this
parameter in GABAB-knockout mice (103.1±1.6 % of
control, n=2 p > 0.05). These
preliminary results show that in the absence of GABAB receptors,
GABA fails to regulate the release of ACh at MOC terminals. We are now evaluating
whether P/Q or N-type Ca2+ channels, that support release at this
synapse, are the targets of GABAB receptor activation.