Understanding the mechanism of silver nanoparticle toxicity.

GARCÉS, MARIANA; MAGNANI, NATALIA; CALABRO, VALERIA; MARCHINI, TIMOTEO; PECORELLI, ALESSANDRA; CACERES, LOURDES; GALDOPORPORA, JUAN; VICO, TAMARA; SALGUEIRO, JIMENA; ZUBILLAGA, MARCELA; MORETTON, MARCELA; DESIMONE, MARTIN; ALVAREZ, SILVIA; VALACCHI, GIUSEPPE; EVELSON, PABLO

Congreso Virtual

Congreso; LXV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2020

Sociedad Argentina de Investigación Clínica

Nanotoxicology has increasingly gained attention along with the AgNP applications development. As inhalation is one of the main routes of exposure, the aim of this study was to evaluate the AgNPharmful effects in the lung. Characterization of AgNP was performed by DLS, TEM and EPR, while biodistribution studies were done using radio labeled 99mTc-AgNP. In vivo studies in balb/c mice intranasally instilled with 0.1 mg AgNP/kg b.w were performed, where O2 metabolism was analyzed 1h after AgNP inhalation. In vitro studies using A549 cells and a 3D lung tissue model, were done after 1h exposure to 2.5 μg AgNP/mL. AgNP showed a hydrodynamic diameter of 17 ± 6 nm and were able to initiate the hemolytic cleavage of H2O2 leading to OH? production. In vivo results showed the lung as the main deposition organ, where AgNP initiate alveolar epithelium barrier injury, revealed by increased protein content and total cell count in BAL samples (46%;120%; p