Time course of inflammatory and oxidative response in environmental particulate matter exposed macrophages

CACERES, LOURDES; PAZ, MARIELA; GARCÉS, MARIANA; CALABRO, VALERIA; MAGNANI, NATALIA; CALTANA, LAURA; TASAT, DEBORAH; ALVAREZ, SILVIA; GONZALEZ MAGLIO, DANIEL; MARCHINI, TIMOTEO; EVELSON, PABLO

Mar del Plata

Congreso; LXIV Reunión Científica Anual; 2019

Sociedad Argentina de Investigación Clínica

Alveolar macrophages are key pulmonary effectors which induce inflammation and oxidative stress after environmental particulate matter (PM) exposure. Therefore, it is of great interest to characterize the oxy-inflammation crosstalk in this scenario. The aim of our work was to study the time course of both oxidative and inflammatory response observed in macrophages after the exposure to Residual Oil Fly Ash (ROFA), a PM surrogate rich in transition metals. The murine cell line RAW 264.7 was exposed to ROFA at 100 μg/mL for 1, 3, 6, and 24 h. Cell viability was not significantly affected under these experimental conditions. Intracytoplasmic complexity was assessed by flow cytometric analysis of light scattering properties (SSC-H) as a cell activation indicator. We found an increase beginning at 1 h time point for the ROFA exposed group (1.00±0.01 vs 1.30±0.02 % control fold change, p