Overexpression of Thioredoxin in heart has a protective effect after exposure to ambient air particles

GUAGLIANONE, ALEJANDRO; GARCÉS, MARIANA; MARCHINI, TIMOTEO; CALABRO, VALERIA; TASAT, DEBORAH; D'ANNUNZIO, VERONICA; GELPI, RICARDO; ALVAREZ, SILVIA; EVELSON, PABLO

Ciudad de Buenos Aires

Congreso; LXIII Reunión Científica Anual; 2018

Sociedad Argentina de Investigación Clínica

The exposure to environmental particulate matter (PM) is associated with increased morbidity and mortality rates due to several cardiovascular diseases, including stroke and myocardial infarction. Thioredoxin (Trx) is a key antioxidant system that plays an important role against oxidative stress; it regulates redox homeostasis and has cardioprotective effects reducing infarct size. A byproduct of fossil fuel combustion known as Residual Oil Fly Ashes (ROFA) is frequently used as a PM surrogate to evaluate the biological effects of the exposure to air pollution. Therefore, the aim of this work was to evaluate the role of Trx after an acute exposure to ROFA and clarify the mechanisms by which PM inhalation triggers cardiovascular adverse health effects. Wild type mice (WT) and transgenic mice overexpressing Trx-1 in heart (TRX) were intranasally instilled with a ROFA suspension (1 mg/kg) or saline solution (control group), and hearts were removed after 3 hours. Heart O2 consumption was significantly increased by 39% in TRXROFA mice compared to WTROFA (WTROFA: 1040 ± 90 ng-at O/min.g tissue, p