Unravelling the relationship between the severity of endotoxemia with mitochondria dysfunction: impact in cardiac function

VICO, TAMARA; VANASCO, VIRGINIA; MARCHINI, TIMOTEO; LORENZETTI, MARIO; ADAN AREAN, JUAN; CALABRO, VALERIA; GARCÉS, MARIANA; VALDEZ, LAURA; FERRERO, MARIANA; GONZALEZ MAGLIO, DANIEL; GINART, SANTIAGO; CORACH, DANIEL; MAZO, TAMARA; D'ANNUNZIO, VERONICA; GELPI, RICARDO; EVELSON, PABLO; ALVAREZ, SILVIA

Lisboa

Congreso; 19th Biennial meeting for the Society for Free Radical Research International (SFRRI); 2018

Society for Free Radical Research International

The aim of this work was to elucidate the relationship between the severity of the systemic inflammation response with cardiac dysfunction and mitochondrial function in endotoxemia and sepsis. Female Sprague-Dawley rats were i.p. injected with LPS (0.5 mg/kg or 8 mg/kg body wt) or vehicle, and after 6 h were euthanized. Blood NO-Hb adduct levels increased by 5-fold after LPS 0.5, and 11-fold after LPS 8. NO production by PMN increased by 42% in LPS 0.5, and 60% in LPS 8 group, while ROS production increased by 90% in both treatments. Impaired cardiac contractile reserve and lusitropic reserve were observed in both LPS groups. However, systemic and local inflammation was only observed after LPS 8. Finally, mitochondrial H2O2 production increased by 40% and 60% in LPS 0.5 and LPS 8 groups (control: 0.128 ± 0.013 nmol/min-mg prot, p