Analysis of the reactive oxygen species sources involved in lung oxidative metabolism after an acute exposure to ambient particulate matter.

MAGNANI, NATALIA; MARCHINI, TIMOTEO; TASAT, DEBORAH; ALVAREZ, SILVIA; EVELSON, PABLO

Londres

Congreso; Society for Free Radical Research International. 16th Biennial Meeting.; 2012

Society for Free Radical Research Inter­national

Reactive oxygen species are able to initiate molecular mechanisms leading to lung oxidative damage due to particulate matter exposure. The aim of this work was to study the O2 metabolism in mice lung after an acute exposure to ambient particles (ROFA) by intranasal instillation (1.00 mg/kg body). Oxygen consumption by tissue cubes was determined by a polarographic technique. As main sources of oxygen uptake, we assessed NADPH oxidase activity in homogenates, following the superoxide anion (O2.-) production and NADPH consumption, and mitochondrial O2 consumption. Respiratory complexes activity and H2O2 were also measured in isolated mitochondria. Measurements were made 1 h after instillation. ROFA exposure showed a 66% increased tissue O2 consumption (control: 225 ± 7 ng-at O/min. g tissue p