Phospholipid multilamellarvesicles entrapping phenothiazine photosensitizers. Preparation, characterization and evaluation of their photodynamic properties

VARA, J.; PERILLO, MA; SANCHEZ, JM; ORTIZ, CS.

JOURNAL OF MOLECULAR LIQUIDS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018

0167-7322

The aim of the present paper was to optimize the properties of phenothiazine photosensitizers (PSs), Azure B and the monobrominated derivative by their entrapment in multilamellar liposomes of egg-phosphatidylcholine(EPC) and dipalmitoylphosphatidylcholine (DPPC). The higher partition coefficient of both PSs in the EPC/water system compared with DPPC/water leaded to select EPC to develop third generation photosensitizer. In addition, the optimal phospholipid/phenothiazine ratio and the location of these dyes in the lipid bilayer systems were established. The entrapment of Azure B and monobrominated Azure B in the multilamellar liposomes decreased the aggregation of the phototherapeutic agents and consequently increased the singlet oxygen quantum yield of the compounds. The use of this vehiculization strategy improved the properties of second generation photosensitizers, enhancing their potential phototherapeutic applications against numerous pathologies.