Effect of sintered time of CaTiO3-CaCO3 in osteoblastic response of KUSA/A1 cells

RODRIGUEZ, ANDREA PAOLA; SANCHEZ, MARÍA ALEJANDRA; TSUJIGIWA, HIDETSUGU; INOUE, MIHO; FELICE, BETIANA; MADRID, ROSSANA ELENA; NAGATSUKA, HITOSHI; FELICE, CARMELO JOSÉ

Paraná, Argentina

Congreso; VI Congreso Latinoamericano de Ingeniería Biomédica; 2014

Sociedad Argentina de Bioingeniería

In recent years, calcium titanate (CaTiO3) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get ?calcium titanate with calcium carbonate? (CaTiO3-CaCO3). Thus, our previous result indicated that calcium carbonate could play a key role in the cell response of CaTiO3 material. In the present study we evaluated the effect of sintering time of CaTiO3-CaCO3 in osteoblastic response of KUSA/A1 cells. We examined the material characterization as well as cellular proliferation and mineralization of KUSA/A1 cells cultured with the materials. The results showed that Ca-TiO3-CaCO3 material sintered during 30 min, has better influ-ence on the bone marrow stromal cells for their proliferation and mineralization compared to CaTiO3-CaCO3 sintered at 2 and 10 h. These results suggest that sintering time could be directly related to the amount of carbon within CaCO3 being crucial in cell response. In conclusion, our preliminary find-ings indicate that CaTiO3-CaCO3 could play a more dominant and favorable effect on cell response when sintered for 30 minutes.