BluBAC system for determining microbial growth in clinical microbiological diagnosis samples by combining photostimulation and turbidimetry

RAMIREZ FROMETA, NARDO CUBANO; LAMOTHE NUVIOLA, CARLOS A. CUBANO; RIVERÓN RODRIGUEZ, ELIER CUBANO; MORENO BARRIOS, CARMEN Y. CUBANO; REGUEIRO GOMEZ, ANGEL CUBANO; FELICE, CARMELO JOSÉ

Biotecnologia Aplicada

Elfo Scientiae

Lugar: La Habana; Año: 2016 vol. 33 p. 2401 - 2405

1027-2852

ABSTRACTAn experimental Workstation was designed, named BluBAC, intended for the microbiological diagnosis in clinical samples, which integrates photostimulation in two zones of the visible light spectrum: red (625 to 644 nm) and blue (430 to 480 nm), together with turbidimetric determinations in biological samples. It allows the analysis of photostimulation on microbial growth. The workstation comprises optoelectronic components and a MSP430 family microcontroller, connected through a Usb port to a computer for processing and visualizing the output signals coming from the samples. The signals are obtained through a graphic control interface in Visual Studio. The influence of the stimulation parameters (wavelength, light intensity, frequency and intensity of stimulation) on the growth of Escherichia coli cells was studied in bacterial cell cultures in DKD medium. The combination of photostimulation with turbidimetric determinations facilitated microbial detection, with decreased lag time and a longer exponential growth phase, stimulating bacterial growth more than other methods previously reported. These results evidenced the potential advantages of the experimental workstation BluBAC over other clinical microbiological diagnostic systems available in the market.