INVESTIGADORES
BOTTINI Ambrosio Ruben
artículos
Título:
A succinate dehydrogenase flavoprotein subunit-like transcript is upregulated in Ilex paraguariensis leaves in response to water deficit and abscisic acid
Autor/es:
ACEVEDO RM, MAIALE SJ, PESSINO SC, BOTTINI R, RUIZ OA, SANSBERRO PA
Revista:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Lugar: Paris; Año: 2013 vol. 65 p. 48 - 54
ISSN:
0981-9428
Resumen:
Ilex paraguariensis plants were subjected to progressive soil water deficit, and differential 33 display (DD) was used to analyse gene expression in leaves to characterise physiological 34 responses to mild and severe water deficits. A cDNA fragment showing strong homology 35 with the flavoprotein subunit (SDH1) of succinate:ubiquinone oxidoreductase (succinate 36 dehydrogenase, SDH, EC 1.3.5.1) was upregulated in plants exposed to drought. 37 Quantitative real-time PCR revealed that the SDH1-like transcript level began to increase 38 when the leaf relative water content (RWC) decreased to 78% and peaked when the RWC 39 dropped to 57%. A correlation between abscisic acid (ABA) concentration and variations in 40 transcript levels was assessed by GC-SIM. After rehydration, SDH1 mRNA and ABA 41 returned to their initial levels. In stressed leaves sprayed with ABA SDH1 mRNA 42 accumulated in greater levels compared to stressed leaves that did not receive ABA. 43 Moreover, the enzymatic activity of succinate dehydrogenase increased 1.5-fold in the 44 mature leaves of ABA-treated plants. This physiological response may be related to the 45 tendency of this species to minimise water losses through stomatal closure in the early 46 stages of dehydration to avoid tissue desiccation. As the leaf water potential diminished due 47 to an increase in water restriction, I. paraguariensis leaf tissues reacted by making osmotic 48 adjustments to sustain tissue metabolic activity, which enables the recovery of 49 photosynthesis upon re-watering. These results provide new insights concerning the linkage 50 between plant respiration and photosynthetic metabolism that could be potentially further 51 used in breeding programs aiming water tolerant genotypes