INVESTIGADORES
CHIMENO ZOTH Silvina Andrea
artículos
Título:
Multiple recombinant ELISA for the detection of anti BVDV antibodies in cattle sera
Autor/es:
SILVINA CHIMENO ZOTH, OSCAR TABOGA
Revista:
JOURNAL OF VIROLOGICAL METHODS
Editorial:
Elsevier Science
Referencias:
Año: 2006 vol. 138 p. 99 - 108
ISSN:
0166-0934
Resumen:
The most immunogenic proteins (E0, E2 and NS3) of BVDV (NADL strain) were expressed in the baculovirus / insect cells system. Recombinant antigens were applied to the design of ELISA tests for the detection of specific antibodies in cattle sera. The developed assays showed to be highly sensitive and specific in comparison with the viral neutralization test, which is the reference test for the serological diagnosis of BVDV. The present results evidence the contribution of each recombinant antigen to clearly determine the pattern of anti-BVDV antibodies in bovine serum samples.
